Procedure for Cleaning, handling and labeling of laboratory glassware.

1.0 OBJECTIVE

To lay down procedure for cleaning, handling and labeling of laboratory glassware.

2.0 SCOPE

This SOP is applicable for cleaning, handling and labeling of laboratory glassware in Company Name

3.0 BACKGROUND

NIL

4.0 RESPONSIBILITY

4.1 All Analytical research personnel are responsible to follow this SOP.

4.2 Group leader of Analytical Research to ensure the procedures to be followed.

4.3 Head Analytical research or his designee to ensure overall compliance.

5.0 PROCEDURE

5.1 Safety

5.1.1 Cleaning of glassware in the laboratory should be done with potable water and thenwith purified water.

5.1.2 Always carry the glassware by using carrier trays / plastic trays.

5.1.3 Always use two hands to hold higher volume glassware, position one hand to hold the bottom.

5.1.4 Never keep hot glassware on a cold bench top.

5.1.5 Never handle broken glassware with bare hands, use cut-resistant gloves.

5.1.6 Never keep glassware at the edges of shelves or platforms.

5.1.7 Don’t let glassware roll around in drawers (use drawer liner pads).

5.1.8 Place glassware well in hoods or on benches.

5.1.9 Examine glassware before use , never use chipped or cracked glassware and never use uncleaned glassware.

5.1.10 Damaged / Broken glassware should be properly disposed in separate bins labeled as “Broken Glassware” (Refer Annexure-1) and enter the details in “Logbook for

Glassware Breakage” (Refer Annexure-2).

5.1.11 Use appropriate PPE while handling various glassware.

5.2 Cleaning of glassware

5.2.1 Preparation of cleaning solution Take 100 ml of cleaning reagent “Extran MA 02 Neutral” Make Merck, into a 10 litre capacity container, add 5 liters of potable water and mix thoroughly to get uniform solution.

5.2.2 Carry the used or contaminated glassware in respective plastic tray to wash area.

5.2.3 Discard the content from glassware and rinse with potable water twice , then soak the glassware in cleaning solution. Ensure that the uncleaned glassware is completely immersed in the cleaning solution.

5.2.4 Soak all glassware for about 30minutes in cleaning solution and take them out. Drain the cleaning solution completely, add little quantity of fresh cleaning solution and add potable water up to half of the capacity and clean them by tilting or shaking vigorously(use nylon brush for cleaning whenever required) and rinse with potable water for two times and finally rinse the glassware with purified water for few more times.

5.2.5 Dry the glassware at 60°C for atleast 1 hour in laboratory oven.

5.2.6 Ensure all the glassware is dried properly and should not contain any traces of water.

5.2.7 Arrange all cleaned and dried glassware in respective places.

5.3 Procedure for cleaning glassware with nitric acid

5.3.1 Clean the glassware with Nitric acid when the apparatus is highly contaminated with organic matter and not cleanable with cleaning solution.

5.3.2 Pour a little quantity of Nitric acid in the glassware which has to be cleaned and keep for 24 hours. After 24 hours discard the added solution and wash with

cleaning solution followed by rinsing with potable water and purified water and then keep for drying.

5.4 Precautions

5.4.1 Handle glassware gently and carefully while washing / cleaning.

5.4.2 Before washing the glassware discard the existing sample solution with proper care. (where ever necessary do proper neutralization)

5.4.3 Collect the broken glassware and hazardous chemicals like KF reagent in separate containers and send it to SHE department.

5.4.4 While washing any glassware wear proper PPE like gloves, goggles, wash coat etc...

5.4.5 Use heat resistant gloves while handling the hot glassware.

5.5 Procedure for handling of glassware

5.5.1 New glassware received should be checked for appropriateness. In case of receipt of any broken glassware the same should be returned to the supplier.

5.5.2 The glassware should be checked for any breakage after drying and brokenglassware should be discarded.

5.5.3 Always carry the glassware by using carrier trays / plastic trays.

5.5.4 Always use two hands to hold higher volume glassware, position one hand ( preferableleft hand ) at the bottom for additional support.

5.5.5 Do not handle the glassware at their neck (e.g. mobile phase bottles, higher volumevolumetric flasks)

5.6 Labeling of Glassware

5.6.1 Labeling should be done by using blue color pen marker.

5.6.2 Label should be clear and legible.

5.6.3 Label should contain the following:

5.6.3.1 Sample / Product./.Material Name

5.6.3.2 A.R No. / Exp. No / Batch No. / Lot No.

5.6.3.3 Preparation Details: For standard preparation it was denoted as STD.

5.6.3.4 For sample preparation it was denoted as SPL.